Commercial Access Only

Specimen SS-COM-YST-0607 // YEAST

PE-2 Y1-PAL+-FD

Saccharomyces cerevisiae

Yeast

PE-2 SeriesTier 1

Target Trait

50

mg/L

Reported target: 50 mg/L capsaicin

Technical Specifications

Genomic Edits

12

GC Content

(41%)

Construct Length

22,100 bp

Promoter System

TEF1/PGK1/GAL1/ADH1 constitutive array

Expression System

Yeast genomic integration (4 loci)

Host Strain

PE-2

Pathway Edits

  • ARO4(K229L) feedback-resistant
  • ARO7(G141S) feedback-resistant
  • ΔARO10
  • ΔPAD1
  • PAL1 overexpression (GAL1 inducible)
  • Enhanced phenylalanine ammonia-lyase flux

Key Interventions

PAL1 overexpression drives phenylpropanoid branchElevated cinnamic acid intermediate for capsaicin

Application Domains

Food ManufacturingIndustrial

Intended Use

High-yield Brazilian industrial strain achieving up to 50 mg/L capsaicin; optimized for food-grade fermentation and industrial capsaicin production..

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