CEN.PK-B Y5-POX--FD

Saccharomyces cerevisiae

Yeast

CEN.PK-B SeriesTier 5

Target Trait

300

mg/L

Reported target: 300 mg/L capsaicin

Technical Specifications

Genomic Edits

GC Content

(41%)

Construct Length

22,900 bp

Promoter System

TEF1/PGK1/GAL1/ADH1 constitutive array

Expression System

Yeast genomic integration (4 loci)

Host Strain

CEN.PK-B

Pathway Edits

ARO4(K229L) feedback-resistant
ARO7(G141S) feedback-resistant
ΔARO10
ΔPAD1
ΔPOX1 (fatty acid beta-oxidation knockout)
Full 9-gene cassette + POX deletion

Key Interventions

POX1 knockout increases acyl-CoA poolImproved vanillylamine acylation efficiency

Application Domains

Food ManufacturingIndustrial

Intended Use

Pharma-grade CEN.PK derivative delivering 300 mg/L capsaicin with low endotoxin profile; designed for food-grade fermentation and industrial capsaicin production..

Access

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