CEN.PK-A Y6-DUAL-FD

Saccharomyces cerevisiae

Yeast

CEN.PK-A SeriesTier 6

Target Trait

400

mg/L

Reported target: 400 mg/L capsaicin

Technical Specifications

Genomic Edits

GC Content

(41%)

Construct Length

25,800 bp

Promoter System

TEF1/PGK1/GAL1/ADH1 constitutive array

Expression System

Yeast genomic integration (4 loci)

Host Strain

CEN.PK-A

Pathway Edits

ARO4(K229L) feedback-resistant
ARO7(G141S) feedback-resistant
ΔARO10
ΔPAD1
Pun1(S39L) variant — enhanced acyltransferase activity
ESM2 chaperone co-expression

Key Interventions

Pun1(S39L) point mutant increases acyltransferase kcatESM2 co-expression stabilizes Pun1 folding

Application Domains

Food ManufacturingIndustrial

Intended Use

Industrial food-grade chassis engineered for 400 mg/L capsaicin output; suited for food-grade fermentation and industrial capsaicin production. with Pun1(S39L) acyltransferase enhancement.

Access

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