BY4741 Y2-MAX-FD

Saccharomyces cerevisiae

Yeast

BY4741 SeriesTier 2

Target Trait

100

mg/L

Reported target: 100 mg/L capsaicin

Technical Specifications

Genomic Edits

GC Content

(41%)

Construct Length

27,400 bp

Promoter System

TEF1/PGK1/GAL1/ADH1 constitutive array

Expression System

Yeast genomic integration (4 loci)

Host Strain

BY4741

Pathway Edits

ARO4(K229L) feedback-resistant (2× copy)
ARO7(G141S) feedback-resistant (2× copy)
ΔARO10, ΔPAD1, ΔPOX1 triple knockout
PAL1 overexpression + Pun1(S39L+L345G)
Full 9-gene cassette + TKL1/TKL2
ESM2 dual chaperone variant

Key Interventions

All knockouts combined with all overexpressionsMaximum-yield configuration for industrial productionDual chaperone + dual Pun1 mutant stack

Application Domains

Food ManufacturingIndustrial

Intended Use

Academic and early-development chassis targeting 100 mg/L capsaicin; ideal for food-grade fermentation and industrial capsaicin production. with maximum combined edits stack.

Access

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