Menthol Bridge T5-AG-RS

Capsicum chinense

Bhut Jolokia

Menthol Bridge SeriesTier 5

Target Trait

2.3

TRPM8/TRPV1 ratio

Reported target: TRPM8/TRPV1 ratio 2.3

Technical Specifications

Genomic Edits

GC Content

(55%)

Construct Length

11,040 bp

Promoter System

Tissue-specific 2A11

Expression System

Agrobacterium-mediated T-DNA

Host Strain

EHA105

Pathway Edits

TRPM8 Y745H gain-of-function
TRPV1 co-regulation
Full biphasic thermosensory axis
Menthol + Icilin co-production

Key Interventions

Menthol and icilin co-production pathway via dual-module terpene synthaseTRPM8/TRPV1 ratio optimization scaffold with feedback-insensitive promoters

Application Domains

Research

Intended Use

Research-grade Menthol Bridge T5 construct; TRPM8/TRPV1 ratio 2.3. Optimized for mechanistic studies of biphasic thermosensation, TRPM8/TRPV1 ion channel co-activation kinetics, dual-receptor pharmacology screening, and cool-burn sensation pathway dissection.

Access

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