Ice-Fire Scale T5-PR-RS

Capsicum annuum

Serrano

Ice-Fire Scale SeriesTier 5

Target Trait

1.8

TRPM8/TRPV1 ratio

Reported target: TRPM8/TRPV1 ratio 1.8

Technical Specifications

Genomic Edits

GC Content

(51%)

Construct Length

10,380 bp

Promoter System

Ubiquitin / T7

Expression System

Protoplast PEG-fusion

Host Strain

PEG-mediated

Pathway Edits

TRPM8 Y745H gain-of-function
TRPV1 co-regulation
Full biphasic thermosensory axis
Menthol + Icilin co-production

Key Interventions

TRPM8 constitutive gain-of-function via S1 helix engineeringTRPV1 co-regulated expression with convergent promoter architecture

Application Domains

Research

Intended Use

Research-grade Ice-Fire Scale T5 construct; TRPM8/TRPV1 ratio 1.8. Optimized for mechanistic studies of biphasic thermosensation, TRPM8/TRPV1 ion channel co-activation kinetics, dual-receptor pharmacology screening, and cool-burn sensation pathway dissection.

Access

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