Ice-Fire Scale T4-BL-RS

Capsicum annuum

Serrano

Ice-Fire Scale SeriesTier 4

Target Trait

1.6

TRPM8/TRPV1 ratio

Reported target: TRPM8/TRPV1 ratio 1.6

Technical Specifications

Genomic Edits

GC Content

(49%)

Construct Length

9,360 bp

Promoter System

CaMV 35S / Ubiquitin

Expression System

Biolistic transformation

Host Strain

Particle Gun

Pathway Edits

TRPM8 gain-of-function
TRPV1 co-expression tuning
Icilin analog synthesis

Key Interventions

TRPM8 constitutive gain-of-function via S1 helix engineeringTRPV1 co-regulated expression with convergent promoter architecture

Application Domains

Research

Intended Use

Research-grade Ice-Fire Scale T4 construct; TRPM8/TRPV1 ratio 1.6. Optimized for mechanistic studies of biphasic thermosensation, TRPM8/TRPV1 ion channel co-activation kinetics, dual-receptor pharmacology screening, and cool-burn sensation pathway dissection.

Access

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