Ice-Fire Scale T4-AG-FD

Capsicum annuum

Serrano

Ice-Fire Scale SeriesTier 4

Target Trait

1.6

TRPM8/TRPV1 ratio

Reported target: TRPM8/TRPV1 ratio 1.6

Technical Specifications

Genomic Edits

GC Content

(49%)

Construct Length

9,360 bp

Promoter System

Tissue-specific 2A11

Expression System

Agrobacterium-mediated T-DNA

Host Strain

EHA105

Pathway Edits

TRPM8 gain-of-function
TRPV1 co-expression tuning
Icilin analog synthesis

Key Interventions

TRPM8 constitutive gain-of-function via S1 helix engineeringTRPV1 co-regulated expression with convergent promoter architecture

Application Domains

Food Manufacturing

Intended Use

Food-grade cool-burn formulation; Ice-Fire Scale T4 at TRPM8/TRPV1 ratio 1.6. Suitable for novel cooling confectionery, thermosensory beverage ingredients, biphasic hot-cold flavor compounds, and dual-sensation spice blends for commercial food manufacturing.

Access

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