Lemongrass T2-LF-FD

Cymbopogon citratus

Lemongrass

SS-LMG SeriesTier 4

Target Trait

500

SHU

Reported target: 500 SHU

Technical Specifications

Genomic Edits

GC Content

(45%)

Construct Length

8,600 bp

Promoter System

EF1α constitutive

Expression System

pLenti-CMV cationic lipid nanoparticle delivery

Host Strain

Cationic lipofection suspension culture LC-50

Pathway Edits

Full 10-gene capsaicinoid pathway in dual attB genomic landing pad
CRISPRi multiplexed silencing of 5 competing metabolic sink genes
PntAB transhydrogenase insertion for NADPH cofactor balance
Central carbon flux redirection via pck/pykA double knockout

Key Interventions

CRISPRi 5-gene metabolic sink silencing via dCas9-KRAB fusionPntAB transhydrogenase NADPH regeneration (200% improvement)AroGfbr feedback-resistant precursor supply (3× phenylalanine pool)

Application Domains

Food ManufacturingAgricultural Biotech

Intended Use

Delivers Lemongrass-platform capsaicinoids at 500 SHU for scalable food-grade heat ingredient manufacturing.

Access

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