Commercial Access Only
Specimen SS-COM-BEY-3016 // BEYOND-PEPPERS
Rosemary T2-PR-RS
Salvia rosmarinus
Rosemary
SS-ROS SeriesTier 4
Target Trait
500
SHU
Reported target: 500 SHU
Technical Specifications
Genomic Edits
12
GC Content
(45%)
Construct Length
8,600 bp
Promoter System
NOS synthetic promoter
Expression System
pKanII PEG-mediated transformation
Host Strain
PEG-mediated protoplast suspension culture
Pathway Edits
- Full 10-gene capsaicinoid pathway in dual attB genomic landing pad
- CRISPRi multiplexed silencing of 5 competing metabolic sink genes
- PntAB transhydrogenase insertion for NADPH cofactor balance
- Central carbon flux redirection via pck/pykA double knockout
Key Interventions
CRISPRi 5-gene metabolic sink silencing via dCas9-KRAB fusionPntAB transhydrogenase NADPH regeneration (200% improvement)AroGfbr feedback-resistant precursor supply (3× phenylalanine pool)
Application Domains
ResearchPharmaceutical
Intended Use
Enables Rosemary cross-species capsaicinoid biosynthesis research and pharmaceutical precursor development at 500 SHU.
Access
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