Commercial Access Only
Specimen SS-COM-BEY-2997 // BEYOND-PEPPERS
Thyme T5-CR-FD
Thymus vulgaris
Thyme
SS-THY SeriesTier 4
Target Trait
10K
SHU
Reported target: 10K SHU
Technical Specifications
Genomic Edits
15
GC Content
(48%)
Construct Length
9,800 bp
Promoter System
AtU6-26 + SpCas9-NG
Expression System
SpCas9-NG base-editor ribonucleoprotein complex
Host Strain
CRISPR-RNP electroporation zygotic embryo
Pathway Edits
- Full 10-gene capsaicinoid pathway in dual attB genomic landing pad
- CRISPRi multiplexed silencing of 5 competing metabolic sink genes
- PntAB transhydrogenase insertion for NADPH cofactor balance
- Central carbon flux redirection via pck/pykA double knockout
Key Interventions
CRISPRi 5-gene metabolic sink silencing via dCas9-KRAB fusionPntAB transhydrogenase NADPH regeneration (200% improvement)
Application Domains
Food ManufacturingAgricultural Biotech
Intended Use
Delivers Thyme-platform capsaicinoids at 10K SHU for scalable food-grade heat ingredient manufacturing.
Access
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