Commercial Access Only
Specimen SS-COM-BEY-2861 // BEYOND-PEPPERS
Basil T2-AG-FD
Ocimum basilicum
Basil
SS-BAS SeriesTier 4
Target Trait
500
SHU
Reported target: 500 SHU
Technical Specifications
Genomic Edits
12
GC Content
(45%)
Construct Length
8,600 bp
Promoter System
FMV 34S promoter
Expression System
T-DNA binary vector pCAMBIA2300
Host Strain
Agrobacterium tumefaciens LBA4404
Pathway Edits
- Full 10-gene capsaicinoid pathway in dual attB genomic landing pad
- CRISPRi multiplexed silencing of 5 competing metabolic sink genes
- PntAB transhydrogenase insertion for NADPH cofactor balance
- Central carbon flux redirection via pck/pykA double knockout
Key Interventions
CRISPRi 5-gene metabolic sink silencing via dCas9-KRAB fusionPntAB transhydrogenase NADPH regeneration (200% improvement)AroGfbr feedback-resistant precursor supply (3× phenylalanine pool)
Application Domains
Food ManufacturingAgricultural Biotech
Intended Use
Delivers Basil-platform capsaicinoids at 500 SHU for scalable food-grade heat ingredient manufacturing.
Access
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