Specimen SS-COM-BEY-2858 // BEYOND-PEPPERS
Basil T1-CR-RS
Ocimum basilicum
Basil
Target Trait
100
SHU
Reported target: 100 SHU
Technical Specifications
Genomic Edits
11
GC Content
(44%)
Construct Length
8,200 bp
Promoter System
U6-26 sgRNA + 35S BE4max
Expression System
SpCas9-NG base-editor ribonucleoprotein complex
Host Strain
CRISPR-RNP electroporation zygotic embryo
Pathway Edits
- Full 10-gene capsaicinoid pathway in dual attB genomic landing pad
- CRISPRi multiplexed silencing of 5 competing metabolic sink genes
- PntAB transhydrogenase insertion for NADPH cofactor balance
Key Interventions
Application Domains
Intended Use
Enables Basil cross-species capsaicinoid biosynthesis research and pharmaceutical precursor development at 100 SHU.
Access
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