Commercial Access Only
Specimen SS-COM-BEY-2686 // BEYOND-PEPPERS
Cacao T4-PR-RS
Theobroma cacao
Cacao
SS-CAC SeriesTier 4
Target Trait
5K
SHU
Reported target: 5K SHU
Technical Specifications
Genomic Edits
14
GC Content
(47%)
Construct Length
9,400 bp
Promoter System
35S CaMV PEG-optimized
Expression System
pKanII PEG-mediated transformation
Host Strain
PEG-mediated protoplast suspension culture
Pathway Edits
- Full 10-gene capsaicinoid pathway in dual attB genomic landing pad
- CRISPRi multiplexed silencing of 5 competing metabolic sink genes
- PntAB transhydrogenase insertion for NADPH cofactor balance
Key Interventions
CRISPRi 5-gene metabolic sink silencing via dCas9-KRAB fusionPntAB transhydrogenase NADPH regeneration (200% improvement)AroGfbr feedback-resistant precursor supply (3× phenylalanine pool)
Application Domains
ResearchPharmaceutical
Intended Use
Enables Cacao cross-species capsaicinoid biosynthesis research and pharmaceutical precursor development at 5K SHU.
Access
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