Commercial Access Only
Specimen SS-COM-BEY-2673 // BEYOND-PEPPERS
Cacao T3-BL-FD
Theobroma cacao
Cacao
SS-CAC SeriesTier 4
Target Trait
1K
SHU
Reported target: 1K SHU
Technical Specifications
Genomic Edits
13
GC Content
(46%)
Construct Length
9,000 bp
Promoter System
CaMV 35S biolistic
Expression System
pAHC17 biolistic expression cassette
Host Strain
PDS-1000/He embryogenic callus DV8
Pathway Edits
- Full 10-gene capsaicinoid pathway in dual attB genomic landing pad
- CRISPRi multiplexed silencing of 5 competing metabolic sink genes
- PntAB transhydrogenase insertion for NADPH cofactor balance
- Central carbon flux redirection via pck/pykA double knockout
- G6PD overexpression NADPH supply module under trc promoter
Key Interventions
CRISPRi 5-gene metabolic sink silencing via dCas9-KRAB fusionPntAB transhydrogenase NADPH regeneration (200% improvement)
Application Domains
Food ManufacturingAgricultural Biotech
Intended Use
Delivers Cacao-platform capsaicinoids at 1K SHU for scalable food-grade heat ingredient manufacturing.
Access
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