Commercial Access Only
Specimen SS-COM-BEY-2664 // BEYOND-PEPPERS
Cacao T2-BL-RS
Theobroma cacao
Cacao
SS-CAC SeriesTier 4
Target Trait
500
SHU
Reported target: 500 SHU
Technical Specifications
Genomic Edits
12
GC Content
(45%)
Construct Length
8,600 bp
Promoter System
Act1 rice promoter
Expression System
pAHC17 biolistic expression cassette
Host Strain
PDS-1000/He embryogenic callus DV8
Pathway Edits
- Full 10-gene capsaicinoid pathway in dual attB genomic landing pad
- CRISPRi multiplexed silencing of 5 competing metabolic sink genes
- PntAB transhydrogenase insertion for NADPH cofactor balance
- Central carbon flux redirection via pck/pykA double knockout
Key Interventions
CRISPRi 5-gene metabolic sink silencing via dCas9-KRAB fusionPntAB transhydrogenase NADPH regeneration (200% improvement)AroGfbr feedback-resistant precursor supply (3× phenylalanine pool)
Application Domains
ResearchPharmaceutical
Intended Use
Enables Cacao cross-species capsaicinoid biosynthesis research and pharmaceutical precursor development at 500 SHU.
Access
Request Commercial Access
This construct is available exclusively to qualified institutions. Submit an inquiry to receive technical documentation and licensing terms.
Contact Us →No pricing is listed publicly. We respond to all qualified inquiries within 2 business days.