Commercial Access Only
Specimen SS-COM-BEY-2650 // BEYOND-PEPPERS
Coffee T5-LF-RS
Coffea arabica
Coffee
SS-COF SeriesTier 4
Target Trait
10K
SHU
Reported target: 10K SHU
Technical Specifications
Genomic Edits
15
GC Content
(48%)
Construct Length
9,800 bp
Promoter System
EF1α constitutive
Expression System
pLenti-CMV cationic lipid nanoparticle delivery
Host Strain
Cationic lipofection suspension culture LC-50
Pathway Edits
- Full 10-gene capsaicinoid pathway in dual attB genomic landing pad
- CRISPRi multiplexed silencing of 5 competing metabolic sink genes
- PntAB transhydrogenase insertion for NADPH cofactor balance
- Central carbon flux redirection via pck/pykA double knockout
Key Interventions
CRISPRi 5-gene metabolic sink silencing via dCas9-KRAB fusionPntAB transhydrogenase NADPH regeneration (200% improvement)
Application Domains
ResearchPharmaceutical
Intended Use
Enables Coffee cross-species capsaicinoid biosynthesis research and pharmaceutical precursor development at 10K SHU.
Access
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