Commercial Access Only
Specimen SS-COM-BEY-2634 // BEYOND-PEPPERS
Coffee T4-BL-RS
Coffea arabica
Coffee
SS-COF SeriesTier 4
Target Trait
5K
SHU
Reported target: 5K SHU
Technical Specifications
Genomic Edits
14
GC Content
(47%)
Construct Length
9,400 bp
Promoter System
GOS2 constitutive
Expression System
pAHC17 biolistic expression cassette
Host Strain
PDS-1000/He embryogenic callus DV8
Pathway Edits
- Full 10-gene capsaicinoid pathway in dual attB genomic landing pad
- CRISPRi multiplexed silencing of 5 competing metabolic sink genes
- PntAB transhydrogenase insertion for NADPH cofactor balance
Key Interventions
CRISPRi 5-gene metabolic sink silencing via dCas9-KRAB fusionPntAB transhydrogenase NADPH regeneration (200% improvement)AroGfbr feedback-resistant precursor supply (3× phenylalanine pool)
Application Domains
ResearchPharmaceutical
Intended Use
Enables Coffee cross-species capsaicinoid biosynthesis research and pharmaceutical precursor development at 5K SHU.
Access
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