Commercial Access Only

Specimen SS-COM-BEY-2528 // BEYOND-PEPPERS

Corn T3-CR-RS

Zea mays

Corn

SS-CRN SeriesTier 4

Target Trait

1K

SHU

Reported target: 1K SHU

Technical Specifications

Genomic Edits

13

GC Content

(46%)

Construct Length

9,000 bp

Promoter System

OsU6 + CBE4max-RNP

Expression System

SpCas9-NG base-editor ribonucleoprotein complex

Host Strain

CRISPR-RNP electroporation zygotic embryo

Pathway Edits

  • Full 10-gene capsaicinoid pathway in dual attB genomic landing pad
  • CRISPRi multiplexed silencing of 5 competing metabolic sink genes
  • PntAB transhydrogenase insertion for NADPH cofactor balance
  • Central carbon flux redirection via pck/pykA double knockout
  • G6PD overexpression NADPH supply module under trc promoter

Key Interventions

CRISPRi 5-gene metabolic sink silencing via dCas9-KRAB fusionPntAB transhydrogenase NADPH regeneration (200% improvement)

Application Domains

ResearchPharmaceutical

Intended Use

Enables Corn cross-species capsaicinoid biosynthesis research and pharmaceutical precursor development at 1K SHU.

Access

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