Commercial Access Only

Specimen SS-COM-BEY-2507 // BEYOND-PEPPERS

Corn T1-CR-FD

Zea mays

Corn

SS-CRN SeriesTier 4

Target Trait

100

SHU

Reported target: 100 SHU

Technical Specifications

Genomic Edits

11

GC Content

(44%)

Construct Length

8,200 bp

Promoter System

U6-26 sgRNA + 35S BE4max

Expression System

SpCas9-NG base-editor ribonucleoprotein complex

Host Strain

CRISPR-RNP electroporation zygotic embryo

Pathway Edits

  • Full 10-gene capsaicinoid pathway in dual attB genomic landing pad
  • CRISPRi multiplexed silencing of 5 competing metabolic sink genes
  • PntAB transhydrogenase insertion for NADPH cofactor balance

Key Interventions

CRISPRi 5-gene metabolic sink silencing via dCas9-KRAB fusionPntAB transhydrogenase NADPH regeneration (200% improvement)

Application Domains

Food ManufacturingAgricultural Biotech

Intended Use

Delivers Corn-platform capsaicinoids at 100 SHU for scalable food-grade heat ingredient manufacturing.

Access

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