Wheat T4-CR-RS

Triticum aestivum

Wheat

SS-WHT SeriesTier 4

Target Trait

SHU

Reported target: 5K SHU

Technical Specifications

Genomic Edits

GC Content

(47%)

Construct Length

9,400 bp

Promoter System

U6-26 sgRNA + 35S BE4max

Expression System

SpCas9-NG base-editor ribonucleoprotein complex

Host Strain

CRISPR-RNP electroporation zygotic embryo

Pathway Edits

Full 10-gene capsaicinoid pathway in dual attB genomic landing pad
CRISPRi multiplexed silencing of 5 competing metabolic sink genes
PntAB transhydrogenase insertion for NADPH cofactor balance

Key Interventions

CRISPRi 5-gene metabolic sink silencing via dCas9-KRAB fusionPntAB transhydrogenase NADPH regeneration (200% improvement)AroGfbr feedback-resistant precursor supply (3× phenylalanine pool)

Application Domains

ResearchPharmaceutical

Intended Use

Enables Wheat cross-species capsaicinoid biosynthesis research and pharmaceutical precursor development at 5K SHU.

Access

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