Commercial Access Only
Specimen SS-COM-BEY-2446 // BEYOND-PEPPERS
Rice T5-PR-RS
Oryza sativa
Rice
SS-RIC SeriesTier 4
Target Trait
10K
SHU
Reported target: 10K SHU
Technical Specifications
Genomic Edits
15
GC Content
(48%)
Construct Length
9,800 bp
Promoter System
NOS synthetic promoter
Expression System
pKanII PEG-mediated transformation
Host Strain
PEG-mediated protoplast suspension culture
Pathway Edits
- Full 10-gene capsaicinoid pathway in dual attB genomic landing pad
- CRISPRi multiplexed silencing of 5 competing metabolic sink genes
- PntAB transhydrogenase insertion for NADPH cofactor balance
- Central carbon flux redirection via pck/pykA double knockout
Key Interventions
CRISPRi 5-gene metabolic sink silencing via dCas9-KRAB fusionPntAB transhydrogenase NADPH regeneration (200% improvement)
Application Domains
ResearchPharmaceutical
Intended Use
Enables Rice cross-species capsaicinoid biosynthesis research and pharmaceutical precursor development at 10K SHU.
Access
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