Commercial Access Only
Specimen SS-COM-BEY-2296 // BEYOND-PEPPERS
Fig T5-PR-RS
Ficus carica
Fig
SS-FIG SeriesTier 3
Target Trait
150K
SHU
Reported target: 150K SHU
Technical Specifications
Genomic Edits
12
GC Content
(46%)
Construct Length
8,600 bp
Promoter System
NOS synthetic promoter
Expression System
pKanII PEG-mediated transformation
Host Strain
PEG-mediated protoplast suspension culture
Pathway Edits
- De novo capsaicin pathway 7-gene cassette insertion via T2A polyprotein
- CYP450 cassette with P450 reductase CPR1 co-expression (1:1 ratio)
- ABC transporter ABCG16 knock-in for vacuolar product export
- Vacuolar sequestration module VSM1 integration
Key Interventions
T2A self-cleaving polyprotein single-cassette deliveryCPR1 co-expression at 1:1 stoichiometry with CYP450 cassette
Application Domains
ResearchPharmaceutical
Intended Use
Enables Fig cross-species capsaicinoid biosynthesis research and pharmaceutical precursor development at 150K SHU.
Access
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