Passion Fruit T2-PR-RS

Passiflora edulis

Passion Fruit

SS-PAS SeriesTier 3

Target Trait

SHU

Reported target: 5K SHU

Technical Specifications

Genomic Edits

GC Content

(43%)

Construct Length

7,400 bp

Promoter System

NOS synthetic promoter

Expression System

pKanII PEG-mediated transformation

Host Strain

PEG-mediated protoplast suspension culture

Pathway Edits

De novo capsaicin pathway 7-gene cassette insertion via T2A polyprotein
CYP450 cassette with P450 reductase CPR1 co-expression (1:1 ratio)
ABC transporter ABCG16 knock-in for vacuolar product export
Vacuolar sequestration module VSM1 integration

Key Interventions

T2A self-cleaving polyprotein single-cassette deliveryCPR1 co-expression at 1:1 stoichiometry with CYP450 cassetteABCG16 vacuolar transporter for sequestration and yield protection

Application Domains

ResearchPharmaceutical

Intended Use

Enables Passion Fruit cross-species capsaicinoid biosynthesis research and pharmaceutical precursor development at 5K SHU.

Access

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