Commercial Access Only
Specimen SS-COM-BEY-2095 // BEYOND-PEPPERS
Grape T5-PR-FD
Vitis vinifera
Grape
SS-GRP SeriesTier 3
Target Trait
150K
SHU
Reported target: 150K SHU
Technical Specifications
Genomic Edits
12
GC Content
(46%)
Construct Length
8,600 bp
Promoter System
NOS synthetic promoter
Expression System
pKanII PEG-mediated transformation
Host Strain
PEG-mediated protoplast suspension culture
Pathway Edits
- De novo capsaicin pathway 7-gene cassette insertion via T2A polyprotein
- CYP450 cassette with P450 reductase CPR1 co-expression (1:1 ratio)
- ABC transporter ABCG16 knock-in for vacuolar product export
- Vacuolar sequestration module VSM1 integration
Key Interventions
T2A self-cleaving polyprotein single-cassette deliveryCPR1 co-expression at 1:1 stoichiometry with CYP450 cassette
Application Domains
Food ManufacturingAgricultural Biotech
Intended Use
Delivers Grape-platform capsaicinoids at 150K SHU for scalable food-grade heat ingredient manufacturing.
Access
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