Commercial Access Only
Specimen SS-COM-BEY-2060 // BEYOND-PEPPERS
Grape T1-LF-RS
Vitis vinifera
Grape
SS-GRP SeriesTier 3
Target Trait
500
SHU
Reported target: 500 SHU
Technical Specifications
Genomic Edits
8
GC Content
(42%)
Construct Length
7,000 bp
Promoter System
CMV immediate-early
Expression System
pLenti-CMV cationic lipid nanoparticle delivery
Host Strain
Cationic lipofection suspension culture LC-50
Pathway Edits
- De novo capsaicin pathway 7-gene cassette insertion via T2A polyprotein
- CYP450 cassette with P450 reductase CPR1 co-expression (1:1 ratio)
- ABC transporter ABCG16 knock-in for vacuolar product export
Key Interventions
T2A self-cleaving polyprotein single-cassette deliveryCPR1 co-expression at 1:1 stoichiometry with CYP450 cassette
Application Domains
ResearchPharmaceutical
Intended Use
Enables Grape cross-species capsaicinoid biosynthesis research and pharmaceutical precursor development at 500 SHU.
Access
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