Specimen SS-COM-BEY-1998 // BEYOND-PEPPERS
Mango T5-CR-RS
Mangifera indica
Mango
Target Trait
150K
SHU
Reported target: 150K SHU
Technical Specifications
Genomic Edits
12
GC Content
(46%)
Construct Length
8,600 bp
Promoter System
AtU6-26 + SpCas9-NG
Expression System
SpCas9-NG base-editor ribonucleoprotein complex
Host Strain
CRISPR-RNP electroporation zygotic embryo
Pathway Edits
- De novo capsaicin pathway 7-gene cassette insertion via T2A polyprotein
- CYP450 cassette with P450 reductase CPR1 co-expression (1:1 ratio)
- ABC transporter ABCG16 knock-in for vacuolar product export
- Vacuolar sequestration module VSM1 integration
Key Interventions
Application Domains
Intended Use
Enables Mango cross-species capsaicinoid biosynthesis research and pharmaceutical precursor development at 150K SHU.
Access
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