Commercial Access Only
Specimen SS-COM-BEY-1978 // BEYOND-PEPPERS
Mango T3-CR-RS
Mangifera indica
Mango
SS-MNG SeriesTier 3
Target Trait
25K
SHU
Reported target: 25K SHU
Technical Specifications
Genomic Edits
10
GC Content
(44%)
Construct Length
7,800 bp
Promoter System
OsU6 + CBE4max-RNP
Expression System
SpCas9-NG base-editor ribonucleoprotein complex
Host Strain
CRISPR-RNP electroporation zygotic embryo
Pathway Edits
- De novo capsaicin pathway 7-gene cassette insertion via T2A polyprotein
- CYP450 cassette with P450 reductase CPR1 co-expression (1:1 ratio)
- ABC transporter ABCG16 knock-in for vacuolar product export
- Vacuolar sequestration module VSM1 integration
- Shikimate pathway amplification via DAHPS feedback-resistant overexpression
Key Interventions
T2A self-cleaving polyprotein single-cassette deliveryCPR1 co-expression at 1:1 stoichiometry with CYP450 cassette
Application Domains
ResearchPharmaceutical
Intended Use
Enables Mango cross-species capsaicinoid biosynthesis research and pharmaceutical precursor development at 25K SHU.
Access
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