Commercial Access Only
Specimen SS-COM-BEY-1272 // BEYOND-PEPPERS
Khat T3-AG-RS
Catha edulis
Khat
SS-KHT SeriesTier 2
Target Trait
50K
SHU
Reported target: 50K SHU
Technical Specifications
Genomic Edits
8
GC Content
(42%)
Construct Length
6,600 bp
Promoter System
RbcS tissue-specific
Expression System
T-DNA binary vector pCAMBIA2300
Host Strain
Agrobacterium tumefaciens EHA105
Pathway Edits
- Phenylpropanoid flux redirection via PheA overexpression
- HCBT overexpression under stress-inducible HSPE promoter
- CoA ligase 4CL tuning with synthetic 5′-UTR hairpin
- MYB31 positive transcriptional regulator insertion
- VAM flux optimization via ACT domain C-terminal truncation
Key Interventions
HCBT 3-copy tandem gene dosage amplificationMYB31 positive regulator overexpression dual-vector system
Application Domains
ResearchPharmaceutical
Intended Use
Enables Khat cross-species capsaicinoid biosynthesis research and pharmaceutical precursor development at 50K SHU.
Access
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