Commercial Access Only

Specimen SS-COM-BEY-1264 // BEYOND-PEPPERS

Khat T2-BL-RS

Catha edulis

Khat

SS-KHT SeriesTier 2

Target Trait

10K

SHU

Reported target: 10K SHU

Technical Specifications

Genomic Edits

7

GC Content

(41%)

Construct Length

6,200 bp

Promoter System

Act1 rice promoter

Expression System

pAHC17 biolistic expression cassette

Host Strain

PDS-1000/He leaf-disc explant

Pathway Edits

  • Phenylpropanoid flux redirection via PheA overexpression
  • HCBT overexpression under stress-inducible HSPE promoter
  • CoA ligase 4CL tuning with synthetic 5′-UTR hairpin
  • MYB31 positive transcriptional regulator insertion

Key Interventions

HCBT 3-copy tandem gene dosage amplificationMYB31 positive regulator overexpression dual-vector systemACT domain C-terminal truncation for reduced allosteric feedback

Application Domains

ResearchPharmaceutical

Intended Use

Enables Khat cross-species capsaicinoid biosynthesis research and pharmaceutical precursor development at 10K SHU.

Access

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