Commercial Access Only

Specimen SS-COM-BEY-1243 // BEYOND-PEPPERS

Belladonna T5-BL-FD

Atropa belladonna

Belladonna

SS-BLD SeriesTier 2

Target Trait

500K

SHU

Reported target: 500K SHU

Technical Specifications

Genomic Edits

10

GC Content

(44%)

Construct Length

7,400 bp

Promoter System

Ubi1 maize constitutive

Expression System

pAHC17 biolistic expression cassette

Host Strain

PDS-1000/He leaf-disc explant

Pathway Edits

  • Phenylpropanoid flux redirection via PheA overexpression
  • HCBT overexpression under stress-inducible HSPE promoter
  • CoA ligase 4CL tuning with synthetic 5′-UTR hairpin
  • MYB31 positive transcriptional regulator insertion

Key Interventions

HCBT 3-copy tandem gene dosage amplificationMYB31 positive regulator overexpression dual-vector system

Application Domains

Food ManufacturingAgricultural Biotech

Intended Use

Delivers Belladonna-platform capsaicinoids at 500K SHU for scalable food-grade heat ingredient manufacturing.

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