Commercial Access Only
Specimen SS-COM-BEY-1232 // BEYOND-PEPPERS
Belladonna T4-AG-RS
Atropa belladonna
Belladonna
SS-BLD SeriesTier 2
Target Trait
200K
SHU
Reported target: 200K SHU
Technical Specifications
Genomic Edits
9
GC Content
(43%)
Construct Length
7,000 bp
Promoter System
NOS synthetic promoter
Expression System
T-DNA binary vector pCAMBIA2300
Host Strain
Agrobacterium tumefaciens EHA105
Pathway Edits
- Phenylpropanoid flux redirection via PheA overexpression
- HCBT overexpression under stress-inducible HSPE promoter
- CoA ligase 4CL tuning with synthetic 5′-UTR hairpin
Key Interventions
HCBT 3-copy tandem gene dosage amplificationMYB31 positive regulator overexpression dual-vector systemACT domain C-terminal truncation for reduced allosteric feedback
Application Domains
ResearchPharmaceutical
Intended Use
Enables Belladonna cross-species capsaicinoid biosynthesis research and pharmaceutical precursor development at 200K SHU.
Access
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