Commercial Access Only

Specimen SS-COM-BEY-1224 // BEYOND-PEPPERS

Belladonna T3-BL-RS

Atropa belladonna

Belladonna

SS-BLD SeriesTier 2

Target Trait

50K

SHU

Reported target: 50K SHU

Technical Specifications

Genomic Edits

8

GC Content

(42%)

Construct Length

6,600 bp

Promoter System

CaMV 35S biolistic

Expression System

pAHC17 biolistic expression cassette

Host Strain

PDS-1000/He leaf-disc explant

Pathway Edits

  • Phenylpropanoid flux redirection via PheA overexpression
  • HCBT overexpression under stress-inducible HSPE promoter
  • CoA ligase 4CL tuning with synthetic 5′-UTR hairpin
  • MYB31 positive transcriptional regulator insertion
  • VAM flux optimization via ACT domain C-terminal truncation

Key Interventions

HCBT 3-copy tandem gene dosage amplificationMYB31 positive regulator overexpression dual-vector system

Application Domains

ResearchPharmaceutical

Intended Use

Enables Belladonna cross-species capsaicinoid biosynthesis research and pharmaceutical precursor development at 50K SHU.

Access

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