Henbane T4-LF-RS

Hyoscyamus niger

Henbane

SS-HNB SeriesTier 2

Target Trait

200K

SHU

Reported target: 200K SHU

Technical Specifications

Genomic Edits

GC Content

(43%)

Construct Length

7,000 bp

Promoter System

CMV immediate-early

Expression System

pLenti-CMV cationic lipid nanoparticle delivery

Host Strain

Cationic lipofection suspension culture LC-50

Pathway Edits

Phenylpropanoid flux redirection via PheA overexpression
HCBT overexpression under stress-inducible HSPE promoter
CoA ligase 4CL tuning with synthetic 5′-UTR hairpin

Key Interventions

HCBT 3-copy tandem gene dosage amplificationMYB31 positive regulator overexpression dual-vector systemACT domain C-terminal truncation for reduced allosteric feedback

Application Domains

ResearchPharmaceutical

Intended Use

Enables Henbane cross-species capsaicinoid biosynthesis research and pharmaceutical precursor development at 200K SHU.

Access

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