Commercial Access Only

Specimen SS-COM-BEY-1183 // BEYOND-PEPPERS

Henbane T4-BL-FD

Hyoscyamus niger

Henbane

SS-HNB SeriesTier 2

Target Trait

200K

SHU

Reported target: 200K SHU

Technical Specifications

Genomic Edits

9

GC Content

(43%)

Construct Length

7,000 bp

Promoter System

GOS2 constitutive

Expression System

pAHC17 biolistic expression cassette

Host Strain

PDS-1000/He leaf-disc explant

Pathway Edits

  • Phenylpropanoid flux redirection via PheA overexpression
  • HCBT overexpression under stress-inducible HSPE promoter
  • CoA ligase 4CL tuning with synthetic 5′-UTR hairpin

Key Interventions

HCBT 3-copy tandem gene dosage amplificationMYB31 positive regulator overexpression dual-vector systemACT domain C-terminal truncation for reduced allosteric feedback

Application Domains

Food ManufacturingAgricultural Biotech

Intended Use

Delivers Henbane-platform capsaicinoids at 200K SHU for scalable food-grade heat ingredient manufacturing.

Access

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