Commercial Access Only
Specimen SS-COM-BEY-1137 // BEYOND-PEPPERS
Mandrake T4-CR-FD
Mandragora officinarum
Mandrake
SS-MDR SeriesTier 2
Target Trait
200K
SHU
Reported target: 200K SHU
Technical Specifications
Genomic Edits
9
GC Content
(43%)
Construct Length
7,000 bp
Promoter System
U6-26 sgRNA + 35S BE4max
Expression System
SpCas9-NG base-editor ribonucleoprotein complex
Host Strain
CRISPR-RNP biolistic leaf meristem
Pathway Edits
- Phenylpropanoid flux redirection via PheA overexpression
- HCBT overexpression under stress-inducible HSPE promoter
- CoA ligase 4CL tuning with synthetic 5′-UTR hairpin
Key Interventions
HCBT 3-copy tandem gene dosage amplificationMYB31 positive regulator overexpression dual-vector systemACT domain C-terminal truncation for reduced allosteric feedback
Application Domains
Food ManufacturingAgricultural Biotech
Intended Use
Delivers Mandrake-platform capsaicinoids at 200K SHU for scalable food-grade heat ingredient manufacturing.
Access
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