Commercial Access Only

Specimen SS-COM-BEY-1031 // BEYOND-PEPPERS

Ashwagandha T4-AG-FD

Withania somnifera

Ashwagandha

SS-ASH SeriesTier 2

Target Trait

200K

SHU

Reported target: 200K SHU

Technical Specifications

Genomic Edits

9

GC Content

(43%)

Construct Length

7,000 bp

Promoter System

NOS synthetic promoter

Expression System

T-DNA binary vector pCAMBIA2300

Host Strain

Agrobacterium tumefaciens EHA105

Pathway Edits

  • Phenylpropanoid flux redirection via PheA overexpression
  • HCBT overexpression under stress-inducible HSPE promoter
  • CoA ligase 4CL tuning with synthetic 5′-UTR hairpin

Key Interventions

HCBT 3-copy tandem gene dosage amplificationMYB31 positive regulator overexpression dual-vector systemACT domain C-terminal truncation for reduced allosteric feedback

Application Domains

Food ManufacturingAgricultural Biotech

Intended Use

Delivers Ashwagandha-platform capsaicinoids at 200K SHU for scalable food-grade heat ingredient manufacturing.

Access

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