Ashwagandha T2-CR-RS

Withania somnifera

Ashwagandha

SS-ASH SeriesTier 2

Target Trait

10K

SHU

Reported target: 10K SHU

Technical Specifications

Genomic Edits

GC Content

(41%)

Construct Length

6,200 bp

Promoter System

AtU6-26 + SpCas9-NG

Expression System

SpCas9-NG base-editor ribonucleoprotein complex

Host Strain

CRISPR-RNP biolistic leaf meristem

Pathway Edits

Phenylpropanoid flux redirection via PheA overexpression
HCBT overexpression under stress-inducible HSPE promoter
CoA ligase 4CL tuning with synthetic 5′-UTR hairpin
MYB31 positive transcriptional regulator insertion

Key Interventions

HCBT 3-copy tandem gene dosage amplificationMYB31 positive regulator overexpression dual-vector systemACT domain C-terminal truncation for reduced allosteric feedback

Application Domains

ResearchPharmaceutical

Intended Use

Enables Ashwagandha cross-species capsaicinoid biosynthesis research and pharmaceutical precursor development at 10K SHU.

Access

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