Commercial Access Only

Specimen SS-COM-BEY-1009 // BEYOND-PEPPERS

Ashwagandha T1-LF-FD

Withania somnifera

Ashwagandha

SS-ASH SeriesTier 2

Target Trait

1K

SHU

Reported target: 1K SHU

Technical Specifications

Genomic Edits

6

GC Content

(40%)

Construct Length

5,800 bp

Promoter System

CMV immediate-early

Expression System

pLenti-CMV cationic lipid nanoparticle delivery

Host Strain

Cationic lipofection suspension culture LC-50

Pathway Edits

  • Phenylpropanoid flux redirection via PheA overexpression
  • HCBT overexpression under stress-inducible HSPE promoter
  • CoA ligase 4CL tuning with synthetic 5′-UTR hairpin

Key Interventions

HCBT 3-copy tandem gene dosage amplificationMYB31 positive regulator overexpression dual-vector system

Application Domains

Food ManufacturingAgricultural Biotech

Intended Use

Delivers Ashwagandha-platform capsaicinoids at 1K SHU for scalable food-grade heat ingredient manufacturing.

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