Bittersweet Nightshade T4-PR-FD

Solanum dulcamara

Bittersweet Nightshade

SS-BTN SeriesTier 2

Target Trait

200K

SHU

Reported target: 200K SHU

Technical Specifications

Genomic Edits

GC Content

(43%)

Construct Length

7,000 bp

Promoter System

35S CaMV PEG-optimized

Expression System

pKanII PEG-mediated transformation

Host Strain

PEG-mediated protoplast suspension culture

Pathway Edits

Phenylpropanoid flux redirection via PheA overexpression
HCBT overexpression under stress-inducible HSPE promoter
CoA ligase 4CL tuning with synthetic 5′-UTR hairpin

Key Interventions

HCBT 3-copy tandem gene dosage amplificationMYB31 positive regulator overexpression dual-vector systemACT domain C-terminal truncation for reduced allosteric feedback

Application Domains

Food ManufacturingAgricultural Biotech

Intended Use

Delivers Bittersweet Nightshade-platform capsaicinoids at 200K SHU for scalable food-grade heat ingredient manufacturing.

Access

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