Commercial Access Only
Specimen SS-COM-BEY-0911 // BEYOND-PEPPERS
Bittersweet Nightshade T2-AG-FD
Solanum dulcamara
Bittersweet Nightshade
SS-BTN SeriesTier 2
Target Trait
10K
SHU
Reported target: 10K SHU
Technical Specifications
Genomic Edits
7
GC Content
(41%)
Construct Length
6,200 bp
Promoter System
FMV 34S promoter
Expression System
T-DNA binary vector pCAMBIA2300
Host Strain
Agrobacterium tumefaciens EHA105
Pathway Edits
- Phenylpropanoid flux redirection via PheA overexpression
- HCBT overexpression under stress-inducible HSPE promoter
- CoA ligase 4CL tuning with synthetic 5′-UTR hairpin
- MYB31 positive transcriptional regulator insertion
Key Interventions
HCBT 3-copy tandem gene dosage amplificationMYB31 positive regulator overexpression dual-vector systemACT domain C-terminal truncation for reduced allosteric feedback
Application Domains
Food ManufacturingAgricultural Biotech
Intended Use
Delivers Bittersweet Nightshade-platform capsaicinoids at 10K SHU for scalable food-grade heat ingredient manufacturing.
Access
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