Commercial Access Only
Specimen SS-COM-BEY-0644 // BEYOND-PEPPERS
Black Nightshade T5-BL-RS
Solanum nigrum
Black Nightshade
SS-BNS SeriesTier 1
Target Trait
1M
SHU
Reported target: 1M SHU
Technical Specifications
Genomic Edits
9
GC Content
(42%)
Construct Length
6,200 bp
Promoter System
Ubi1 maize constitutive
Expression System
pAHC17 biolistic expression cassette
Host Strain
PDS-1000/He leaf-disc explant
Pathway Edits
- CHS promoter swap for enhanced phenylpropanoid flux
- CYP450-B1 integration at native PAL locus
- PAL1 cDNA overexpression cassette (35S:PAL1)
- 4CL2 knockout to redirect coumaroyl-CoA toward capsaicinoid branch
Key Interventions
CS overexpression at 12× native baseline via enhanced 35SPAL1 promoter swap to constitutive CaMV 35S enhancer
Application Domains
ResearchPharmaceutical
Intended Use
Enables Black Nightshade cross-species capsaicinoid biosynthesis research and pharmaceutical precursor development at 1M SHU.
Access
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