Commercial Access Only

Specimen SS-COM-BEY-0633 // BEYOND-PEPPERS

Black Nightshade T4-BL-FD

Solanum nigrum

Black Nightshade

SS-BNS SeriesTier 1

Target Trait

500K

SHU

Reported target: 500K SHU

Technical Specifications

Genomic Edits

8

GC Content

(41%)

Construct Length

5,800 bp

Promoter System

GOS2 constitutive

Expression System

pAHC17 biolistic expression cassette

Host Strain

PDS-1000/He leaf-disc explant

Pathway Edits

  • CHS promoter swap for enhanced phenylpropanoid flux
  • CYP450-B1 integration at native PAL locus
  • PAL1 cDNA overexpression cassette (35S:PAL1)

Key Interventions

CS overexpression at 12× native baseline via enhanced 35SPAL1 promoter swap to constitutive CaMV 35S enhancerCYP450-B1 co-factor regeneration via AtCPR overexpression

Application Domains

Food ManufacturingAgricultural Biotech

Intended Use

Delivers Black Nightshade-platform capsaicinoids at 500K SHU for scalable food-grade heat ingredient manufacturing.

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