Commercial Access Only
Specimen SS-COM-BEY-0611 // BEYOND-PEPPERS
Black Nightshade T2-AG-FD
Solanum nigrum
Black Nightshade
SS-BNS SeriesTier 1
Target Trait
50K
SHU
Reported target: 50K SHU
Technical Specifications
Genomic Edits
6
GC Content
(39%)
Construct Length
5,000 bp
Promoter System
FMV 34S promoter
Expression System
T-DNA binary vector pCAMBIA2300
Host Strain
Agrobacterium tumefaciens EHA105
Pathway Edits
- CHS promoter swap for enhanced phenylpropanoid flux
- CYP450-B1 integration at native PAL locus
- PAL1 cDNA overexpression cassette (35S:PAL1)
- 4CL2 knockout to redirect coumaroyl-CoA toward capsaicinoid branch
Key Interventions
CS overexpression at 12× native baseline via enhanced 35SPAL1 promoter swap to constitutive CaMV 35S enhancerCYP450-B1 co-factor regeneration via AtCPR overexpression
Application Domains
Food ManufacturingAgricultural Biotech
Intended Use
Delivers Black Nightshade-platform capsaicinoids at 50K SHU for scalable food-grade heat ingredient manufacturing.
Access
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