Commercial Access Only

Specimen SS-COM-BEY-0610 // BEYOND-PEPPERS

Black Nightshade T1-LF-RS

Solanum nigrum

Black Nightshade

SS-BNS SeriesTier 1

Target Trait

5K

SHU

Reported target: 5K SHU

Technical Specifications

Genomic Edits

5

GC Content

(38%)

Construct Length

4,600 bp

Promoter System

CMV immediate-early

Expression System

pLenti-CMV cationic lipid nanoparticle delivery

Host Strain

Cationic lipofection suspension culture LC-50

Pathway Edits

  • CHS promoter swap for enhanced phenylpropanoid flux
  • CYP450-B1 integration at native PAL locus
  • PAL1 cDNA overexpression cassette (35S:PAL1)

Key Interventions

CS overexpression at 12× native baseline via enhanced 35SPAL1 promoter swap to constitutive CaMV 35S enhancer

Application Domains

ResearchPharmaceutical

Intended Use

Enables Black Nightshade cross-species capsaicinoid biosynthesis research and pharmaceutical precursor development at 5K SHU.

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