Commercial Access Only

Specimen SS-COM-BEY-0579 // BEYOND-PEPPERS

Litchi Tomato T3-LF-FD

Solanum sisymbriifolium

Litchi Tomato

SS-LTO SeriesTier 1

Target Trait

250K

SHU

Reported target: 250K SHU

Technical Specifications

Genomic Edits

7

GC Content

(40%)

Construct Length

5,400 bp

Promoter System

SV40 early promoter

Expression System

pLenti-CMV cationic lipid nanoparticle delivery

Host Strain

Cationic lipofection suspension culture LC-50

Pathway Edits

  • CHS promoter swap for enhanced phenylpropanoid flux
  • CYP450-B1 integration at native PAL locus
  • PAL1 cDNA overexpression cassette (35S:PAL1)
  • 4CL2 knockout to redirect coumaroyl-CoA toward capsaicinoid branch
  • CS gene cluster insertion at euchromatic safe-harbor locus

Key Interventions

CS overexpression at 12× native baseline via enhanced 35SPAL1 promoter swap to constitutive CaMV 35S enhancer

Application Domains

Food ManufacturingAgricultural Biotech

Intended Use

Delivers Litchi Tomato-platform capsaicinoids at 250K SHU for scalable food-grade heat ingredient manufacturing.

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