Specimen SS-COM-BEY-0578 // BEYOND-PEPPERS
Litchi Tomato T3-CR-RS
Solanum sisymbriifolium
Litchi Tomato
Target Trait
250K
SHU
Reported target: 250K SHU
Technical Specifications
Genomic Edits
7
GC Content
(40%)
Construct Length
5,400 bp
Promoter System
OsU6 + CBE4max-RNP
Expression System
SpCas9-NG base-editor ribonucleoprotein complex
Host Strain
CRISPR-RNP biolistic leaf meristem
Pathway Edits
- CHS promoter swap for enhanced phenylpropanoid flux
- CYP450-B1 integration at native PAL locus
- PAL1 cDNA overexpression cassette (35S:PAL1)
- 4CL2 knockout to redirect coumaroyl-CoA toward capsaicinoid branch
- CS gene cluster insertion at euchromatic safe-harbor locus
Key Interventions
Application Domains
Intended Use
Enables Litchi Tomato cross-species capsaicinoid biosynthesis research and pharmaceutical precursor development at 250K SHU.
Access
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