Garden Huckleberry T2-PR-FD

Solanum scabrum

Garden Huckleberry

SS-GHU SeriesTier 1

Target Trait

50K

SHU

Reported target: 50K SHU

Technical Specifications

Genomic Edits

GC Content

(39%)

Construct Length

5,000 bp

Promoter System

NOS synthetic promoter

Expression System

pKanII PEG-mediated transformation

Host Strain

PEG-mediated protoplast suspension culture

Pathway Edits

CHS promoter swap for enhanced phenylpropanoid flux
CYP450-B1 integration at native PAL locus
PAL1 cDNA overexpression cassette (35S:PAL1)
4CL2 knockout to redirect coumaroyl-CoA toward capsaicinoid branch

Key Interventions

CS overexpression at 12× native baseline via enhanced 35SPAL1 promoter swap to constitutive CaMV 35S enhancerCYP450-B1 co-factor regeneration via AtCPR overexpression

Application Domains

Food ManufacturingAgricultural Biotech

Intended Use

Delivers Garden Huckleberry-platform capsaicinoids at 50K SHU for scalable food-grade heat ingredient manufacturing.

Access

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