Commercial Access Only
Specimen SS-COM-BEY-0446 // BEYOND-PEPPERS
Tree Tomato T5-PR-RS
Cyphomandra betacea
Tree Tomato
SS-TRT SeriesTier 1
Target Trait
1M
SHU
Reported target: 1M SHU
Technical Specifications
Genomic Edits
9
GC Content
(42%)
Construct Length
6,200 bp
Promoter System
NOS synthetic promoter
Expression System
pKanII PEG-mediated transformation
Host Strain
PEG-mediated protoplast suspension culture
Pathway Edits
- CHS promoter swap for enhanced phenylpropanoid flux
- CYP450-B1 integration at native PAL locus
- PAL1 cDNA overexpression cassette (35S:PAL1)
- 4CL2 knockout to redirect coumaroyl-CoA toward capsaicinoid branch
Key Interventions
CS overexpression at 12× native baseline via enhanced 35SPAL1 promoter swap to constitutive CaMV 35S enhancer
Application Domains
ResearchPharmaceutical
Intended Use
Enables Tree Tomato cross-species capsaicinoid biosynthesis research and pharmaceutical precursor development at 1M SHU.
Access
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