Tree Tomato T4-CR-RS

Cyphomandra betacea

Tree Tomato

SS-TRT SeriesTier 1

Target Trait

500K

SHU

Reported target: 500K SHU

Technical Specifications

Genomic Edits

GC Content

(41%)

Construct Length

5,800 bp

Promoter System

U6-26 sgRNA + 35S BE4max

Expression System

SpCas9-NG base-editor ribonucleoprotein complex

Host Strain

CRISPR-RNP biolistic leaf meristem

Pathway Edits

CHS promoter swap for enhanced phenylpropanoid flux
CYP450-B1 integration at native PAL locus
PAL1 cDNA overexpression cassette (35S:PAL1)

Key Interventions

CS overexpression at 12× native baseline via enhanced 35SPAL1 promoter swap to constitutive CaMV 35S enhancerCYP450-B1 co-factor regeneration via AtCPR overexpression

Application Domains

ResearchPharmaceutical

Intended Use

Enables Tree Tomato cross-species capsaicinoid biosynthesis research and pharmaceutical precursor development at 500K SHU.

Access

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